The Oxford BRC provides core support for a dedicated team of scientists who manage and run the Diabetes and Metabolism Theme laboratory. The lab performs a wide range of biochemical and genomic assays for theme investigators as well as supporting human islet isolation and assessment of human islet quality for clinical and research use. We have a dedicated mass spectrometry unit where metabolic tracers (stable-isotope labelled fatty acids and other molecules) used in our human in vivo studies are analysed, and a number of core platforms, including Envision and MSD plate readers an ILab 650 Clinical Chemistry analyser, ABI 7900s and confocal microscopes.
Thomas Clague – BRC Diabetes & Metabolism Lab Manager
As the BRC Diabetes & Metabolism Lab Manager, I head up a small core team (Alexandra Soca, Sarah Larkin) in OCDEM that perform a wide range of biochemical and genomic assays for theme investigators, as well as supporting human islet isolation and assessment of human islet quality for clinical and research use.
BRC Core Lab Team
Throughout my research career I have gained practical expertise in immunohistochemistry/ immunofluorescence, macromolecule extraction, archiving and quality control, PCR/qRT-PCR, immunoblotting, digital pathology image analysis, confocal microscopy, HPLC, mass spectrometry, primary and continuous cell culture, cloning and microtomy.
My favourite part is working on multiple projects at the same time: from nucleic acid extractions to lipid biochemistry and islet isolations and QC, I move from lab to lab and help different groups with their research.